Abstract

PHYTOCHEMICAL STUDY AND ANTIBACTERIAL ACTIVITY OF ETHANOLIC AND AQUEOUS EXTRACTS OF LEAVES OF Myrianthus holstii ENGL. (CECROPIACEAE)

Silue Kalamourou, Doumbia Idrissa*, Yeo Sounta Oumar, Yapi Houphouet Felix, N’guessan Jean David and Djaman Allico Joseph

Abstract

This study aims to perform the phytochemistry study and evaluate the antibacterial effects of aqueous extracts and ethanolic macerated leaves of Myrianthus holstii Engl. (Cecropiaceae) on five strains derived from biological products and three reference strains. Phytochemical analysis revealed the presence of polyphenols, flavonoids, quinones, tannins and sterols-polyterpenes in both extracts. The diffusion and dilution methods on Muller-Hinton made it possible to evaluate the antibacterial activity of the extracts. The results revealed that these extracts have a dose-dependent antibacterial activity on the bacterial strains used. However, the 70% ethanolic extract has a better antibacterial potential on the strains compared to the aqueous extract namely on S. aureus (MIC = 3.12 mg / mL), E. coli ATCC (MIC = 12.5 mg / mL) and S. aureus Meti-R (MIC = 6.12 mg / mL). The diameters of inhibition are between 08 and 18 mm for the ethanolic extract from 25 mg / ml and between 08 and 14 mm for the aqueous extract from 50 mg / ml. The MIC of the aqueous extract vary from 12.5 to 100 mg / mL and those of the ethanol extract range from 3.12 to 50 mg / mL. The extracts are bactericidal on all the strains after 24 and 48 hours of incubation. This study showed that Myrianthus holstii extract could be used in the treatment of infectious diseases.

Keywords: Myrianthus holstii; Phytochemistry; Antibacterial Activity.