Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING, ISOLATION AND STRUCTURAL ELUCIDATION OF BIOACTIVE COMPOUND FROM METHANOL BARK EXTRACTS OF FICUS POLITA

Zannah Zakariya Goni* and Musa Maikudi Idriss

Abstract

The aim of this study was to screen the phytochemical constituents, isolate and elucidate the structure of bioactive compound from methanol extracts from the bark of Ficus polita. The qualitative phytochemical analysis of the bark extract of Ficus polita was done following standard procedures and the tests revealed the presence of saponins, tannins, steroids, triterpenoids and lipids. The methanol extract of the bark led to the isolation of one compound, E5. Structure determination was accomplished by means of spectroscopic methods (FT-IR, 13C, 1H, COSY, and HSQC NMR). The spectral data of the isolated compound E5 is identical with spectral data of literature study, the spectral interpretation of the compound E5 shows that it is a cardiolipin derivative, is a kind of disphosphatidylglycerol lipid in which all four of the phosphatidyl acyl groups are specified as oleoyl, it has two phosphatidic acid moieties connected to glycerol backbone in the center and potentially carries two negative charges, and is elucidated to be Tetraoleoylcardiolipin (C81H150O17P2) which is reported for the first time from Ficus polita.

Keywords: Ficus polita, Methanol extract, Bark, Cardiolipin, Tetraoleoylcardiolipin.