Abstract

DETERMINATION OF THE OPTIMUM CONCENTRATION OF SNCL2.2H2O AS A REDUCING AGENT IN THE LABELING OF APIGENIN COMPOUNDS WITH RADIONUCLIDE TECHNETIUM- 99M AS A RADIOTRACER COMPOUND FOR CANCER DIAGNOSIS

Danni Ramdhani*, Maula Eka Sriyani, Marisa Dwi Ariani, Eva Maria Widyasari

Abstract

Objective: The purpose of this study is to determine the optimization of the amount of SnCl2.2H2O reducing agent in the process of labeling Apigenin flavonoid compounds with Technetium-99m radionuclide compounds. The compound formed is expected to be a radiotracer compound for cancer diagnosis. Methods: The method used in optimizing the 99mTc-Apigenin compound labeling is the number of SnCl2.2H2O reducing agents. The optimum reducing agent concentration can be evaluated from the value of obtaining the best radiochemical purity value. Results: The results of optimization of the number of SnCl2.2H2O reducing agents on the synthesis of 99mTc- Apigenin compound obtained the optimum concentration of SnCl2.2H2O is 0.02 mg with a radiochemical purity value of 74.88% ± 1.86%. Conclusion: The optimum amount of SnCl2.2H2O reducing agent is 0.02 mg for 99mTc-Apigenin labeling, with the least amount of 99mTcO4 - and TcO2 impurities at 21.99%±2.02% and 3.13%±0.49%.

Keywords: Apigenin, reducting agent, SnCl2.2H2O, Technetium-99m, Radiochemical Purity, 99mTc-Apigenin, Radiotracer.