Abstract

COMPARATIVE ANALYSIS OF HAEMATINIC EFFECT OF DHATRYARISTA WITH STANDARD FERROUS SULPHATE IN TANNIC ACID INDUCED IRON DEFICIENCY ANAEMIA IN ALBINO WISTAR RATS

Srivastava V., Sarkar S.*, Pattanayak C., Mishra M. and Jena J.

Abstract

Background: Iron deficiency anemia affects more than 1.2 billions individuals worldwide, and iron deficiency in the absence of anemia is even more frequent. Iron deficiency is caused by physiologically increased iron requirements in children, adolescents, young and pregnant women, by reduced iron intake, or by pathological defective absorption or chronic blood loss. Treatment modalities include both oral and parenteral therapies. Objectives: a) To study the improvement in haemoglobin after giving dhatryarista in IDA. b) To compare the effect of Dhatryarista (in different concentrations) with standard oral formulation Ferrous sulphate in terms of haemoglobin. Materials and methods: The study was conducted for the period of two months in department of Pharmacology KIMS, bhubaneswar. Total 24 Albino wistar rats were taken and divided into 4 groups containing 6 rats each. Anaemia was induced by adding tannic acid in diet of 24 rats by 21days. After induction of anaemia, Group II, Group III and Group IV rats were given standard drug (Ferrous sulphate), Dhatryarista sample 1 and Dhatryarista sample 2 respectively, alongwith normal diet for next 30 days. Blood samples were taken in day 1, day 21 and day 53. Results: All the values are expressed as a Mean ± SEM (standard error of mean).The data were analyzed by one way ANOVA and post hoc methods. using socscistatistics.com. Significant decrease in haemoglobin were observed, when compared between day 1 and day 21, while significant increase in haemoglobin was observed after 30 days of administration of test and standard drug, when compared between day 21 and day 53. Conclusion: The administration of tannic acid to rats caused anaemia characterized by reducing hematological parameters. The oral administration of Dhatryarista in the dose of 4.32ml/kg/day P.O and 8.64ml/kg/day P.O significantly increased haemoglobin level. The results demonstrated that higher doses of the plant extract didnot show any signs of acute toxicity in animals.

Keywords: Iron deficiency anaemia, Dhatryarista, Ferrous sulphate, Amalaki, Haemoglobin.