Abstract

COMPARATIVE STUDY OF ANTIDIABETIC ACTIVITY OF ZINC OXIDE NANOPARTICLES AND GLIPIZIDE IN STREPTOZOTOCINE INDUCED DIABETIC WISTAR RATS

Raut Shrish V., Gauri Rajendra Kulkarni, Kavita Dhar Bagati and *Neerjesh

Abstract

Background: Nanopharmacology has exceeded the pace of pharmacological and toxicological research on the effects of nanoparticles in the biological environment. The behavior of the Pharmacological parameters of nanoparticle in biological systems still require much investigation before they can be effectively utilized in human treatment paradigms. Aims: To compare safety and antidiabetic activity of Glipizide and ZnONP in Streptozotocin - induced diabetic Wistar rats. Settings and Design: Total 36 adult, male wistar rats of comparable developmental age and weight; were incorporated in the study in six groups. Induction of Type 2 DM was done in 30 animals by administration of Streptozotocin 45 mg/kg subcutaneously. Methods and Material: Group one and two animals were administered with normal saline and group three was administered with Glipizide at a dose of 5 mg/kg/day/animal. Group four, five and six received ZnONP at a dose of 10 mg/kg, 50 mg/kg and 100 mg/kg respectively/day per animal. Group six also received Glipizide at a dose of 5 mg kg/day/animal. Animal were treated till 28 days and then blood samples were tested for hematology, biochemistry. Selected organs were sent for histopathology. Statistical analysis used: ANOVA. Results: There is additional weight reduction effect of ZnONP with higher doses of ZnONP more antiobesity activity. Persistently low BSL were achieved by group six which received both ZnONP and Glipizide as treatment and Group four which received ZnONP (10 mg/kg) only shows steady fall in BSL levels. Combination of ZnONP (100 mg/kg) with Glipizide has maximum reduction in BSL indicating combination has achieved highest glycemic control as compared. All groups receiving ZnONP as a treatment have shown almost similar smooth glycemic control on OGTT avoiding acute hypoglycemic or hyperglycemic episodes. Conclusions: ZnONPs have affinity towards pancreatic tissue and ZnONP (< 50 nm) show antidiabetic activity at graded concentration. When administered along with oral antidiabetic drug, ZnONP have shown added antidiabetic effect.

Keywords: Antidiabetic, Zinc oxide, Nanoparticles