Abstract

OPTIMIZATION OF APIGENIN LIGAND IN THE 99MTC-APIGENIN COMPOUND SYNTHESIS PROCESS AS A POTENTIAL RADICAL SCAVENGER AGENT

Danni Ramdhani*, Maula Eka Sriyani, Marisa Dwi Ariani, Eva Maria Widyasari

Abstract

Objective. The purpose of this study is to find the optimum conditions of apigenin ligands in the 99mTc-Apigenin compound synthesis process. The antioxidant activity of apigenin can be used to detect the presence of excess free radicals in the body. The compound formed is expected to be a radiotracer compound for cancer diagnosis. Methods. The method used in the synthesis of 99mTc-Apigenin compounds is the amount of apigenin ligands. The optimal concentration of apigenin ligand can be evaluated from the acquisition value of the best radiochemical purity. Results. The results of optimization of the number of Apigenin ligands in the synthesis of 99mTc-Apigenin compounds obtained optimal apigenin concentration was 0.5 mg with a radiochemical purity value of 85,93%±1,10%. Conclusion. The optimal amount of apigenin ligand is 0.5 mg in the synthesis reaction of 99mTc-Apigenin compound, with the least amount of 99mTcO4 - and TcO2 impurities at 4.14%±0.03% and 9.94%±1.12%.

Keywords: Apigenin, ligand, technetium-99m, radiochemical purity, 99mTc-apigenin, radiotracer.