Abstract

DETECTION OF MULTI DRUG RESISTANT AND EXTENDED SPECTRUM ß-LACTAMASES PRODUCING KLEBSIELLA SPECIES IN KHARTOUM STATE, SUDAN - 2019

Sahar Mohammed Seed Ahmed* and Hassan Abd Alaziz Musa

Abstract

Klebsiella species; are commensal of the gastrointestinal tracts of human and animal. Klebsiella pneumoniae is the commonest human pathogen, known to be resistant to the beta lactam agents, by the production of Extended Spectrum Beta Lactamases (ESBLs). This study was conducted to detect the Multi drug resistant and ESBLs Klebsiella spp in Sudanese patients using phenotypic method. A total number of 300 Klebsiella spp were included. Antibiotic sensitivity was done, 79 of isolates showed multi drug resistance. The multi drug resistant strain were further identified using API 20 E system. Twenty five out of the 79 MDR strains were identified as K. pneumoniae (22) and K. oxytoca (3). Phenotypic detection for ESBL was positive result for the 25 isolates. The antibiotic resistance in the 25 isolate was very high for most of the used antibiotics. They were sensitive to colistin (96%) and imipenem (92%). and all MDR Klebsiella spp. As conclusion the commonest isolates of MDR and ESBLs producer in Sudan was Klebsiella pneumoniae & Klebsiella oxytoca. They were resistant to most of the used antibiotics except imipenem and colistin.

Keywords: Extended Spectrum ß- Lactamase (ESBL), Multi Drug Resistant (MDR), Analytical Profile Index(API).