Abstract

PRODUCTION OF NOVEL AMYLASE ENZYME FROM HALICONA FIBULATA ASSOCIATED MARINE BACTERIA AGAINST UTI PATHOGEN

Dr. A. Mohankumar*, A. Chithira and S. Vijayalakshmi

Abstract

Today, urinary tract infection is a major health problem affecting pregnancy women and its manifestation persists throughout the life despite treatment, it is a multifactorial disease which is caused by host agents and environmental factors. The UTI infection caused by Escherichia coli also most common pathogen in human life, is also serious problem among UTI patients. So midstream urine samples were collected from different Govt. Hospital in and around Tirupur District. A total of 20 multidrug resistant strains were isolated from 50 urine samples and were analyzed and characterized. Further, the antimicrobial susceptibilities of this isolates was assessed against 8 commonly used antibiotics, Nalidixic acid (5mcg), Cefotaxime (5mcg), Clindamycin (10mcg), Vancomycin (30mcg), Ampicillin (10mcg), Chloramphenicol (10mcg), Tetracycline (30mcg) and Ceftriaxone (30mcg). Among these strains 70%, 80%, 50%, 75%, 70%, 10%, 40% and 85% were found to be exhibit a significance degree of resistance to four groups of antibiotics. Currently, the development of bacterial resistance to presently available antibiotics has necessitated the search for new antibacterial agents. During the last two decade, several studies have been performed to establish biologically active products from marine sources, which mostly deals with marine bacteria and their potential role in the production of metabolites is becoming thrust area for research, sponges have been recognized as a rich source of novel compounds that are of potential interest to mankind for diagnosis urinary tract infection. In this study marine sponge Halicona fibulata were isolated and identified using biochemical characterization. Further, the associated microbe recovered from the sample and it was checked for production of amylase enzyme. The selected colony ability to produce the amylase enzyme and it was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography. Finally the different concentration of purified amylase enzyme 50μg, 100μg, and 150μg tested against the E. coli. The maximum zone of inhibition (22 mm) was observed against the isolate MT07 at 150μl. The minimum zone (16 mm) was recorded in the strain MT08. In this study sponge derived amylase enzyme was used and it shows prominent antimicrobial activity against UTI pathogen. Thus an alternative strategy targeting the endosymbiosis bacteria of sponges for the screening of bioactive natural products may prove to be an effective approach to come to blows emerging multi resistant clinical pathogens.

Keywords: UTI pathogen, Antibiotic susceptibility, Marine Sponge, Amylase enzyme.