Abstract

EVALUATION OF ENZYMATIC AND NON-ENZYMATIC ANTIOXIDANT ACTIVITIES OF IN VIVO AND IN VITRO REGENERATED ALOE VERA L.

Neelofar Khanam*, and G.K. Sharma

Abstract

Since ancient time a great number of aromatic, medicinal, spice and other plants contain chemical compounds exhibiting antioxidant properties, such as their secondary metabolites, which play an important role in protecting the cell or tissues from oxidative damage. In the present investigation in vitro enzymatic and non-enzymatic antioxidant activities in the leaves of in vivo and in vitro regenerated Aloe vera L. were tested. The plant leaves were analysed for the selected enzymatic antioxidants such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), glutathione S-transferase (GST), polyphenol oxidase (PPO), and for the non-enzymatic antioxidants such as ascorbic acid, tocopherol, total carotenoids, total phenols, reduced glutathione, flavonoids were examined. Results were showing that the leaves from in vitro propagated Aloe vera L. were having higher level of antioxidants when compared to antioxidant levels in the leaves of in vivo grown Aloe vera L.

Keywords: Aloe vera L., in vitro and in vivo, enzymatic antioxidants, non-enzymatic antioxidants.