Abstract

EVALUATION OF RENOPROTECTIVE ACTIVITY OF MESOZEAXANTHIN IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Shaveta Bhardwaj*, Tapan Behl, Kulwinder Singh, Pyare Lal Sharma

Abstract

Diabetic nephroopathy (DN) is one of the most common complications of a common prevalent chronic endocrine disorder – diabetes mellitus. It involves a great deal of damage to the renal anatomy and physiology. The following mentioned study was undertaken to investigate the renoprotective effect, if any, of mesozeaxanthin, an antioxidant in experimentally induced diabetic nephropathy in rats. A single dose of Streptozotocin (STZ) (50 mg/kg, i.p.) was incorporated to precipitate the induced-condition of diabetes mellitus in the concerned rats. Diabetic nephropathy developed after 8 weeks of STZ administration and was assessed by the measurement of various parameters viz., levels of serum creatinine, blood urea nitrogen (BUN) and creatinine clearance, renal collagen content. Furthermore, changes in renal TBARS (Thiobarbituric acid reactive substances) and reduced glutathione levels were measured as markers of oxidative stress besides measuring the levels of glucose and lipid content for also observing the possibility of the drug action via some other therapeutic activity. Mesozeaxanthin treatment for the first time has been demonstrated to significantly attenuate STZ-induced DN, as evidenced by a significant decrease in serum creatinine, BUN, renal collagen content and a significant increase in creatinine clearance. Mesozeaxanthin treatment dose-dependently decreased the renal oxidative stress in diabetic rats. It is, thus, concluded that the renoprotective effect of mesozeaxanthin is due to its antioxidant property as no significantchanges were observed in blood glucose and lipid profile, confirm that mesozeaxanthin is a pure antioxidant.

Keywords: diabetic nephropathy, reactive oxygen species, oxidative stress, serum creatinine, blood urea nitrogen.