Abstract

ANTIMICROBIAL EFFECT OF LEAVE EXTRACT OF GUIERA SENEGALENSIS ON AEROBIC BACTERIA ISOLATED FROM DIABETIC FOOT INFECTION IN KHARTOUM STATE, SUDAN

*Sara M. Ali

Abstract

Comparison of in vitro susceptibility of bacteria to commonly used antimicrobial drug and Guiera senegalensis which is used as traditional drug in treatment of diabetes mellitus in Sudan was carried out. In this prospective cross-sectional descriptive study, the clinical data and specimens were obtained from 50 patients in three Hospitals in Khartoum state during the period from Mar 2016 to July 2016. The specimens were processed using bacteriology isolation & identification techniques. Antimicrobial susceptibility and antibacterial activity testing were carried out using the disc diffusion and cup plate method. Growth was obtained from all the specimens. Pseudomonas aeruginosa (20%) and Proteus mirabilis was (17%) while Proteus vulgaris nd Klebsiella pneumoniae gave (15%) each and Escherichia coli (11%), Klebsiella oxytoca and Citrobacter koseri had (2%) each. On the other hand the gram positive Staphylococcus aureus had (15%) while Enterococcus faecalis (3%). The antimicrobial susceptibility pattern shows amikacin as effective antibiotic against gram negative bacteria while vancomycin as effective antibiotic against gram positive organism. Pseudomonas aeruginosa was highly resistant to most of the antibiotic, seconded by Proteus mirabilis then Proteus vulgaris, Staphylococcus aureus, Klebsiella pneumoniae, Klebsiella oxytoca, Enterococcus faecalis and Citrobacter koseri. The methanol and residual extract of the leave Guiera senegalensis showed effective antimicrobial activity against various pathogenic bacteria Screening, proper evaluation, early identification and the proper selection of antibiotics for treatment of the ‘at risk foot’ is important in management of the Diabetic foot infection.

Keywords: Diabetic Foot, Guiera senegalensis, antimicrobial effect.