Abstract

SIMULTANEOUS QUANTITATIVE DETERMINATION OF ATENOLOL AND AMLODIPINE BESILATE IN THEIR COMBINED DOSAGE FORM BY USING UV SPECTROPHOTOMETER

Devi Velmurugan*, Ilavarasan K., Lavanya R., Sandhiya P., Sekar V. and Sambathkumar R.

Abstract

To develop a simple, sensitive and highly accurate Simultaneous Estimation of Atenolol and Amlodipine Besilate in their bulk and pharmaceutical combined tablet dosage forms. 0.1N NaOH used as a solvent. Then spectra of Atenolol and Amlodipine Besilate exhibit at λ max of 232nm and 236nm respectively. The linearity of calibration curve (Absorbance Vs Concentration) in pure solution was checked over the concentration range of about 4-24μg/ml for Atenolol and 5-30 μg/ml for Amlodipine Besilate respectively. With the correlation coefficient higher than 0.99. The developed method was validated in terms of accuracy, precision, linearity, ruggedness, robustness, limit of detection and limit of quantification which proves suitability of proposed method for routine estimation of Atenolol and Amlodipine Besilate on bulk pharmaceutical formulation. The methods were validated as per ICH guidelines.

Keywords: Amlodipine Besilate; Atenolol; Sodium hydroxide; distilled water, UV Spectrophotometer; validation.