Abstract

COMPARATIVE ANALYSIS OF ANTIMICROBIAL ACTIVITY OF AQUEOUS ETHANOLIC AND CHLOROFORM EXTRACTS OF POLYGONATUM CIRRHIFOLIUM

*Reenu Chauhan, Roma Ghai, K. Nagarajan, Kalaiwani, K. Anil Kumar Teotia, Garima Varshney and Charanpreet Kaur

Abstract

The aim of this study is to determine antimicrobial activity of aqueous ethanol (5:5) and chloroform extracts of rhizomes of Polygonatum cirrhifolium by agar well diffusion method. Organisms used for testing antimicrobial activity are Salmonella typhi, Kocuria rhizophila, Bacillus subtilis, Klebsiella pneumonia, Staphylococcus aureus, Psedomonas aerugenosa, Escherichia coli, Candida albicans, Saccromyces cervisiae. Agar well diffusion method was used to determine antimicrobial investigation of rhizomes of Polygonatum cirrhifolium. Aqueous ethanol extract of P.cirrhifolium displays significant zone of inhibition of Staphylococcus aureus (23.2mm), Bacillus subtilis (26.1mm), Kocuria rhizophila (17.5mm), Salmonella typhi (25.7mm), Klebsiella pneumonia (9.2mm), Psedomonas aerugenosa (34.8mm) whereas chloroform extract zone of inhibition against Kocuria rhizophila (16.1 mm), Staphylococcus aureus (18.3 mm), Bacillus subtilis (20.9mm), Salmonella typhi (22.4 mm), Klebsiella pneumonia (8.4 mm), Psedomonas aerugenosa (40.6 mm) at 5 μg/ml. Maximum inhibition in aqueous ethanol as well as chloroform extract were shown in the case of Psedomonas aerugenosa, microbe which is a gram negative bacteria. However, inhibition zone was nil in case of fungi Candida albicans and Saccromyces cervisiae. Results from the study suggest that aqueous ethanol of P.cirrhifolium shows better antibacterial action. This can be used as the alternative to currently available antibacterial drugs and can be used for burn and wound infection.

Keywords: Extracts, Polygonatum cirrhifolium, rhizome, well diffusion method, antimicrobial activity DMSO (Dimethylsulphoxide).