Abstract

STUDY OF ASSOCIATIVE SOLVATION OF ISONIAZID WITH BOVINE SERUM ALBUMIN AND GLYCINE IN AQUEOUS MEDIUM THROUGH VOLUMETRIC AND ACOUSTIC ANALYSIS

Ashok Kumar Sahoo, Sunil Kumar Pradhan, Ajay Kumar Patnaik and Smrutiprava Das*

Abstract

Measurements of Density (ρ), speed of sound (u), and viscosity (η) of a pharmacologically significant anti-tuberculosis drug Isoniazid with a simplest amino acid Glycine and a poly amino acid Bovine serum albumin (BSA) has been carried out in aqueous medium at normal body temperature of 370C and atmospheric pressure. An Aston par De nsimeter, Ostwald viscometer, ultrasonic interferometer are used in the concentration range (2 x 10-4 to 2 x 10-3M) at 310K. The experimental data has been meticulously used to enumerate various parameters which assist to predict valuable information regarding the presence of different kinds of intermolecular interactions possible in both the ternary systems. The results lead to comparative study of associative parameters of (Isoniazid + water + Glycine) vs. (Isoniazid + water + BSA) systems. The experimental outcomes were also interpreted in terms of various volumetric & acoustic parameters such as adiabatic compressibility (βs), intermolecular free length (Lf), apparent molar volume (φv), apparent molar compressibility (φκ), specific acoustic impedance (Z), relative association (RA) etc to establish solute-solvent and solute-solute interactions. The UV –VIS Study justifies the difference in interaction between GLY -BSA and INZ-BSA systems in term of ion-solvent and ion-ion interaction in aqueous medium. Hydrogen bonding and weak van der-Waals attraction are the key players in ternary system and it is observed that these interactions are of higher order when we transit from INZ-GLY system to INZ-BSA system.

Keywords: Isoniazid, Glycine, Bovine Serum Albumin, Ultrasonic velocity, adiabatic compressibility, Intermolecular free length, Relative association.