Abstract

IN VIVO PROTECTIVE EFFECTS OF MURRAYA KOENIGII LEAF EXTRACT AGAINST CYCLOPHOSPHAMIDE INDUCED MICRONUCLEI IN BONE MARROW CELLS OF MICE

Ravi Prasad A. and *Rudrama Devi K.

Abstract

In the present study the genetic protective nature of Murraya koenigii leaf extract were carried out in Cyclophosphamide CP) induced micronuclei in polychromatic erythrocytes of mice. Two experiments were conducted in Swiss mice. In one experiment the animal were administered with various doses of curry leaves as 100mg/kg, 200mg/kg and 400mg/kg to assess the anti-mutagenic nature of leaf extract in another experiment the priming the animals with two doses of curry leaf extract and intraperitoneal injection of CP 50mg/kg to experimental animal. all animals were sacrificed after24 hours and slides prepared and screened for the presence of micronuclei in bone marrow cells of mice. when given curry leaf extract the percentage of micronuclei were lower no significant incidence was observed however when primed with curry leaf extract in CP induced genetic damage there was a reduction was noted indicating protective nature of leaf extract. Hence the data clearly indicate use of curry leaf extract is essential in chemotherapy regimen.

Keywords: Murraya koenigii leaf extract, Cyclophosphamide, micronuclei, mice.