Abstract

PHYSICOCHEMICAL, PHYTOCHEMICAL SCREENING AND PROFILING OF SECONDARY METABOLITES OF ANNONA SQUAMOSA LEAF EXTRACT

Vanitha Varadharajan*, Umadevi Kumba Janarthanan, Vijayalakshmi Krishnamurthy

Abstract

Annona squamosa is a traditional plant predominantly seen in Tamilnadu, India. The principle objective of the study is to assess the phytochemicals present in the ethanolic leaf extract of Annona squamosa, prepared from organic solvents of ascending polarity index (Petroleum ether < Chloroform < Ethanol < Aqueous) and to analyse the bioactive principles in the ethanolic crude extract by TLC, HPLC and HPTLC. Phytochemical analysis revealed the presence of carbohydrates, alkaloids, flavonoids, tannins, terpenoids, quinones and glycosides in the ethanolic leaf extract. TLC profiling of ethanolic leaf extracts reveals the presence of phytochemicals. Different Rf (Retention factor) value of various phytochemicals provide valuable infomation regarding their polarity and selection of solvents for separation of phytochemicals. HPLC revealed the presence of Rutin, Quercetin, Kamepherol, Farmarixetin, and Isorhamnetin in the ethanolic leaf extract. Researches in bioactive substances might lead to the discovery of new compounds that could be used to formulate new and most potent antimicrobial drugs to overcome the problem of resistant to the currently available antibiotic.

Keywords: Annona squamosa, Phytochemicals, HPLC, HPTLC, Physicochemical.