Abstract

PHYTOCHEMICAL ANALYSIS OF CALOTROPIS GIGANTEA (LINN) AND IT'S BIOLOGICAL ACTIVITIES

Dr. Amutha Swaminathan*, Deepika Krishnamoorthi, Lavanya Nallasamy and Dr. Karthiyaini Ramasamy

Abstract

Calotropis gigantea Linn. belongs to the family Asclepiadaceae, is commonly known as giant milkweed. It has a lot of medicinal properties which cures many diseases. The aim of this present study is to standardize qualitative preliminary phytochemical screening to analyse secondary metabolites present in this plant. It was carried out from flower extract obtained by sequential method using organic solvents such as hexane, chloroform, ethyl acetate and methanol. Carbohydrates, alkaloids, and phenolic compounds such as flavonoids, terpenes, were present. The antioxidant activity was performed with this four extracts, which take place in DPPH assay of TLC spot test method. The extracts obtained from Calotropis gigantea were analyzed for anticancer activity against breast cancer cell line. It can be carried out by calorimetric assay known as MTT assay using a dye compound. The in vitro cytotoxicity showed the maximum cell viability in HEX (67.49 μg/mL) and MEOH (71.30 μg/mL) extracts. Cytotoxic potential against Breast cancer cell line (MDAMB231) with IC50 value of hexane and methanol extracts obtained 84.05 μg/mL and 72.27 μg/mL respectively.

Keywords: Medicinal properties, phytochemical analysis, Phenolic compound, TLC, antioxidant, anticancer activity.