Abstract

IMPACT OF DOXORUBICIN AND VITAMIN E, A SEPARATELY AND IN COMBINATION ON SELECTED ANTIOXIDANT ENZYME ACTIVITIES OF RAT MYOCARDIAL TISSUE

K. Harish Babu*, B. Vijayudu and K. Vijayakumari

Abstract

The present study investigated the in vivo effects of 15 mg/kg wt of Doxorubicin and 100 mg/kg wt of Vitamin E, 50 IU/kg wt of Vitamin A separately and in combination over 8 weeks (weekly doses) on myocardial antioxidant enzyme activity levels in rats. After 8 weeks the activities of Superoxide dismutase, Catalase, Glutathione reductase, Glutathione-S-transferase, Glutathione peroxidase and the levels of Lipid peroxides and reduced Glutathione were presented. The data indicated a significant decrease in all the parameters studied under Doxorubicin stress. Doxorubicin altered parameters were significantly reversed or recovered by vitamin E plus Doxorubicin. Vitamin A plus doxorubicin also significantly recovered the Doxorubicin altered parameters except Catalase and Glutathione peroxidase activities. From the results it is reported that Doxorubicin impair overall antioxidant enzyme activities and the antioxidants like vitamin E and vitamin A by their antioxidant mechanism might be neutralizing the Doxorubicin altered antioxidants in vivo.

Keywords: Doxorubicin, Vitamin E, Vitamin A, Antioxidant enzymes, Cardiac tissue.