Abstract

HAPLOID PLANT PRODUCTION FROM A POTENTIAL MEDICINAL PLANTS, CALOTROPIS GIGANTEA (LINN)

Dr. Amutha Swaminathan*, Deepika Krishnamoorthi, Lavanya Nallasamy and Dr. Karthiyayini Ramasamy

Abstract

Calotropis gigantea Linn. belongs to the family Asclepiadaceae, is commonly known as giant milkweed which has significant medicinal properties. Tissue culture is a part of our economy, pharmaceutical, food and emerging Biotechnology industries. The aim of this study is to standardize haploid plant production from pollinium and callus production from leaf, node explants cultured on MS medium supplemented with various combinations and concentrations of plant growth hormone such as 2,4 Dichlorophenoxy acetate (2,4 D) and Indole 3- acetic acid (IAA). Callus induction of the leaf explants of IAA of (0.5 mg/mL) showed the maximum 94.2% of callus. When compared to the other hormones such as 2, 4-D showed 94.2% and callus induction of 2,4-D + IAA showed 94.3%. Nodal explant showed maximum 81.7% on MS medium supplemented with 2,4-D + IAA (0.5mg/mL) when compared to the IAA and 2,4-D and it showed 77.2% and 77.0% of callus induction respectively. Pollinium explants showed 92.2% maximum growth on MS medium supplemented with 2,4-D (0.5mg/mL). IAA and 2,4-D + IAA showed 84.6% and 92% of callus induction respectively.

Keywords: Calotropis gigantea, Callus induction, Haploid plant production, Pollinium.