Abstract

PHARMACOGNOSTICAL AND PHARMACEUTICAL ANALYSIS OF KHADIRADI GHANAVATI- AN AYURVEDIC FORMULATION FOR DIABETES MELLITUS (TYPE 2)

Pankaj Kaushik*, Alankruta R. Dave, C. R. Harisha, V. J. Shukla and Ritu

Abstract

Introduction: Khadiradi Ghanavati, is formulation of Khadira, Kadara and Puga. This preparation is given in Madhumeha by Chakradatta. Though the combination had been suggested in the form of decoction in the classical text, it is converted into the tablet (Ghanavati) form for resolving palatability issues and proper dose fixation in terms of its effectivity. The pharmacognostical and physico-chemical data is not published on Khadiradi Ghanavati till date. Hence the present study was planned. Aim: To evaluate the pharmacognostical and physicochemical characters of Khadiradi Ghanavati. Materials & Methods: The finished product is collected from pharmacy of the institute and authenticated from the laboratory of the same institute. Standard procedures were followed to undertake pharmacognostical and physico-chemical analysis. The Khadiradi Ghanavati subjected to microscopic evaluation, physico-chemical analysis like hardness, loss on drying, ash value, pH value, weight variation in tablet, water soluble extract, alcohol soluble extract, High Performance thin layer chromatography (HPTLC) etc. Results: Pharmacognostical study showed the presence of certain identifying characters of all of the three ingredients in the preparation like epicarp cells of Puga, fibres of Kadar, prismatic crystals of Khadir, scleroids of Kadar and Khadir, silica deposition of Khadir, stone cells of Khadir and Puga and tannin content of Puga etc. Preliminary physico-chemical analysis showed that hardness 3.83 Kg/cm2, ash value 6.10%, loss on drying 16.27%, water soluble extract 30.63%, methanol soluble extract 26.96% and HPTLC showed sixteen spots at 254nm and ten spots at 366nm. Discussion: The Pharmacognostical study comprised of organoleptic study and microscopic study of finished product alongwith physico-chemical analysis finds out authenticity of ingredient of the Khadiradi Ghanavati. Presence of all raw content in the finished product shows genuineness of the product. Conclusion: Presented work was carried out to standardize the finished product Khadiradi Ghanavati in terms of its identity, quality and purity. Pharmacognostical and Physico-chemical observations as there is no reported study till date. It showed the specific characters of all active constituents in the formulation and will be helpful as reference guidance for future scientific evaluation of the drug.

Keywords: Khadiradi Ghanavati, Madhumeha, pharmacognosy, physico-chemical analysis, HPTLC.