Abstract

EFFECT OF CYNODON DACTYLON ON FREE RADICAL LOAD OF LYMPHOID ORGANS OF ASIAN CATFISH, CLARIAS BATRACHUS

Mishra, P.* Jurry, R., and Gupta, S.

Abstract

The aim of the present study was to asses the effect of aqueous and alcoholic extract of Cynodon dactylon on free radical load of lymphoid organs viz., spleen, thymus and head kidney of Asian catfish, Clarias batrachus. For this 36 fishes of mixed sexes were randomly divided into three group; each group consists of 12 fishes. Group one treated as control and group 2 and 3 treated as test receiving 50 and 100 ppm of aqueous or alcoholic extract of Cynodon dactylon every alternate day till 28 days. Test and control fishes were sacrificed after 7, 14, 21 and 28 days and lymphoid organs were collected in icecold containers for enzyme assay. The Cynodon dactylon treated fish showed significant increase in superoxide dismutase and catalase and decrease in lipid peroxidation. No mortality was observed at these doses. Result of ANOVA for SOD & CAT revealed a statistically highly significant main effect of treatment (T), duration (D) of experimentation and interaction effect of treatment with duration (TXD). The result of ANOVA for LPO also showed statistically significant results except alcoholic extract in thymus where duration of experimentation and interaction effect (TXD) was not significant and aqueous extract in spleen where interaction effect (TXD) was not significant.

Keywords: Cynodon dactylon, Clarias batrachus, aqueous & alcoholic extract, antioxidant enzyme, free radicals, lymphoid organs.