Abstract

IMMUNOSUPPRESSIVE POTENTIAL OF PLECTRANTHUS AMBOINICUS LEAF EXTRACT IN CARASSIUS AURATUS

Dayana Janakiraman* and Parameswari C. S.

Abstract

Objective: Leaf extract of Plectranthus amboinicus (PA) possess pharmaceutically important phytoconstituents that contribute to significant curative credential of the plant. The objective of the study was to analyse the Immunosuppressive nature of Hydroalcoholic extract of Plectranthus amboinicus (HAPA) by haematological and other related studies in comparison with immunosuppressive and immunostimulatory standards Cyclosporine A (CsA) and Concanavaline A (ConA) respectively. Methods: Hematological studies and immunomodulatory assays were performed using EAHAPA fraction of the plant. Results: Hematological indices such as RBC, WBC, Differential count, showed significant immunosuppressive potential on fishes, whereas Hb, plasma protein and immunoglobin levels were markedly unaffected by EAHAPA fraction. Immunomodulatory assays – carbon clearance assay, Respiratory burst activity, Neutrophil activity and myeloperoxidase activity showed significant immunosuppressive credential on par with CsA. Conclusion: The immunomodulatory studies and hematological indices analysis have revealed that innate immune system of the animal is affected. Further studies on animal model would reveal specific molecular targets involved in the action of plant extract for employing it in the treatment of autoimmune disorders.

Keywords: Immunosuppression, Hematology, Plectranthus, Myeloperoxidase, Autoimmune disorder.