Abstract

PHYTOCHEMICAL SCREENING AND TLC PROFILE OF ETHYL ACETATE EXTRACT OF LEUCAS BIFLORA LEAVES

Karishma R. Chakole*, Kamala K. Chandak and Milind. J. Umekar

Abstract

The present study was aimed to carry out preliminary phytochemical screening and thin layer chromatography of Ethyl acetate extract of Leucas biflora leaves. The plant has been reported to be used traditionally for the treatment of conjunctivitis, nosebleed and white discharge in women. The plant material was collected, leaves were shade dried and pulverized into coarse powder. It was then extracted with Ethyl acetate solvent by soxhlation. The extract was subjected to preliminary phytochemical screening to detect the presence of primary and secondary metabolites. It showed the presence of Carbohydrate, Lipids, Tannins, Flavonoids and Steroids. A number of different solvent system were tried for developing TLC profile of Ethyl acetate extract of L.biflora leaves and the better separation of the components was observed in Methanol: n-Butanol: Formic acid (8:1:1) solvent system. 5 components were found to be separated with corresponding Rf values 0.109, 0.581, 0.727, 0.927 and 1.00. In future, these phyto-constituents can be isolated by column chromatography and can be characterized by Mass spectroscopy, Infrared spectroscopy, Nuclear magnetic resonance spectroscopy etc. Their pharmacological potential can be evaluated and can be formulated into a suitable dosage form.

Keywords: Leucas biflora, Ethyl acetate, TLC, Phytochemical screening.