Abstract

IN VITRO AND IN VIVO EFFECT OF THREE AQUEOUS PLANT EXTRACT ON PATHOGENICITY OF KLEBSIELLA PNEUMONIA ISOLATED FROM PATIENT WITH URINARY TRACT INFECTION

Sura hsan Abed Chabuck*, Nagham Adil Ghani Chabuck

Abstract

This study was aimed to evaluate the antibacterial activity of four aqueous plants extract (pomegranate, apple, lemon & orange peel) on the survival and virulence factor of Klebsiella pneumonia isolated from urine in vitro and in vivo. Antimidrobial assay relied on the estimation of the colony forming unit number present the pomegranate was higher effect than a anther plant. when study the hidrophobicity of cells precent plant extract have different effect from very strong hydrophopic (apple , lemon ) to hydrophilic (orange) . Bacterial strain loss their ability of hemagglutination in present lemon and apple but orange & pomegranete extracts did not show any alteration of hemagglutination. In curli expression thes found only bacteria incubated in the presence of all concentrations of pomegranete extract and 8000mg/mL of orange& lemon extracts formed white colonies which indicated the loss of curli fimbriae. Other extracts did not inhibit the synthesis of curli fibers. The antihemolytic activities were obtained in the present of pomegranate in 500 mg/ml and orange peels in concentration 1000 mg/ml and higher. Bacteria growing in the presence of apple extracts did not show effect on heamolytic. Bacteria growing in the presence of apple, lemon and pomegranet extracts did not show effect on viscosity in concentration 62.5mg/ml. The histopathological change in mice kidney infect with bacterial suspension only is acute cellular degeneration and degenerated glomeruli and severe degeneration of renaltubules aggregation of inflammatory cells and bleeding in the renal tubule while mice kidney infected with bacterial suspension and pomegranate not observed any histopathological change.

Keywords: antihemolytic, antihemolytic, hemagglutination.