Abstract

ETHANOPHARMACOLOGICAL INVIVO STUDY OF GUL E GHAFIS (FLOWERS OF GENTIANA DAHURICA FISCH) IN FARTETADDASSUMFIDAM (DYSLIPEDEMIA)

Sehba Samreen*, Dr. Mir Yousuf Ali and Dr. Zaibunnisa Begum

Abstract

Gul e ghafis (Gentiana dahurica fisch) has an important place in Unani system of Medicine and it is used in various preparations. According to many unani physicians it is used as bitter tonic, demulcent, stomachic, deobstruent, diaphoretic etc. Latest researches reveal its Hypolipedemic activity. Modern research shows a significant protective effect as an anticonvulsant. Objective: To observe the Hypolipedemic effect of Gul e Ghafis (Flowers of Gentiana dahurica Fisch) with bodyweights and biochemical parameters in diet induced Hyperlipidemia. Methods: 36 female SD rats of 80–100 gm were divided into normal control group (n = 6), HFD group (n=6), LDTD group (n = 6), IDTD group (n = 6), HDTD group (n= 6) and STD group (n = 6) till 12 weeks. Dyslipidaemia were induced in the SD rats by high fat diet (Indian Vanaspathi ghee + Coconut oil in 3: 1 ratio) for 12 weeks. The Group II, III, IV, V and VI were given HFD for 8 weeks to induce dyslipidaemia. Body weights of SD rats were monitored on every 2nd week and Serum Lipid profile (Serum Cholesterol, HDL, LDL & Triglycerides) were done on last day of 8th week which showed the induction of Dyslipidaemia by HFD till 8th week. After 8 weeks of induction the Group III received standard comparative drug i.e. atorvastatin 10 mg/kg/bw and Group IV,V & VI received the HA extract of Gul e Ghafis (Flowers of Gentiana dahurica Fisch) which is carried out till 12 weeks. At the end of the study, the SD rats of Group II, III, IV, V and VI were sacrificed and histopathological assessment was done. Results: The incidence of dyslipidaemia till 8th week in Group II, III, IV, V and VI in terms of marked increase in body weights and also in biochemical parameter i.e. SLP (Serum Total Cholesterol, HDL, LDL and Triglycerides) were observed. Then in the 12th week, a significant decrease in the body weight in Group III (STD group) and Group VI (HDTD) in comparison with Group II (HFD) was observed. In addition a significant decrease in SLP of Group III and Group VI and histopathological structural changes and damage in the Group II (HFD), Group IV (LDTD) and Group V (INTD) are seen. Conclusion: HA extract of Gul e Ghafis can decrease the incidence of obesity and improves the biochemical parameters which may be one of the mechanisms in the treatment of CAD.

Keywords: Hypolipedemic effect, hydro alcoholic extract, Cholesterol and High fat diet.