Abstract

ISOLATION AND PHYTOCHEMICAL CHARACTERIZATION OF ALKALOIDS FROM CLEOME GYNANDRA LINN. AERIAL PARTS

Priyanka B. Ambre and *Dr. Dinesh P. Hase

Abstract

The plant Cleome gynandra L. (Cappariaceae) is commonly known as “Hurhur”, “Tilvan” in Marathi. Traditionally the whole plant is use in the treatment of Tumor, anti-inflammatory, malaria, piles, and rheumatism. The presence of alkaloid was tested qualitatively by dragon drops method. Then it was subjected to quantitative estimation by UV-spectroscopy method. The concentration of 20, 40, 60, 80, 100μg/ml. The absorbance at UV- spectroscopy was observed in 470 nm. Then the isolated alkaloids were subjected for IR spectroscopy. The ranges of IR 3233.23 (N-H st), 2943.49 (C-H st), 2806.78 (C-H st), 2148.88(C-D st), 1644.57(C=C st), 1440.43(CH3 sy), 1346.97(CH2 st), 1193.15(C-H st). The total alkaloids were isolated from methanol extract of Cleome gynandra. The preliminary phytochemical screening was performed and shows the presence of alkaloid, Flavonoid, phenols, carbohydrate and tannins. The isolated alkaloids confirmed by the preliminary phytochemical test of alkaloid. Further the concentration of alkaloid was determined using UV-spectroscopy method and the Total flavonoid and phenolic content was also determined.

Keywords: Cleome gynandra Linn, Isolation of alkaloids, Alkaloid content, Flavonoid content and Phenolic content.