Abstract

DETERMINATION OF TOTAL PHENOLIC, FLAVONOID CONTENT AND ANTIOXIDANT ACTIVITY OF LEUCAENA LEUCOCEPHALA EXTRACT

Vipin Kumar Verma* and Salma Malik

Abstract

Present study was done to evaluate the anti-oxidant and anti-bacterial potential as well as total flavonoid and phenolic content of leaf and pod cover extracts of L. leucocephala. Free radical scavenging activity was determined by 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) Assay and reducing power by Ferric Reducing Ability of Plasma (FRAP) Assay. Total flavonoid was determined by using Dowd’s methods while total phenolic content by Folin-Ciocalteu’s method. Anti-bacterial activity activity was investigated through disc diffusion assay. Methanolic and ethanolic leaf extract showed better anti-oxidant and anti-bacterial potential than the pod cover methanol and ethanol extract. Furthermore, there was increased flavonoid and phenolic content in the leaf extract. Thus, we infer that due to the presence of secondary metabolites (phenol and flavonoids) in the leaf extract, it can serve as a potential source of natural anti-oxidant and anti-bacterial agent.

Keywords: Leucaena leucocephala, Anti-bacterial, Anti-oxidant, Flavonoid, Phenolic.