Abstract

IN VITRO NEUROPROTECTIVE POTENTIAL OF CRESCENTIA CUJETE LEAVES AGAINST MPTP- INDUCED TOXICITY IN SHSY5Y NEUROBLASTOMA CELLS

Anitha P.* and Dr. Nazeema T. H.

Abstract

Objective: Crescentia Cujete are used traditionally to treat neurological disorders. The aim of this study was to evaluate the invitro neuroprotective potential of the plant, after induction of toxicity using MPTP, in SH-SY5Y neuroblastoma cells. Methods: In the present study, it is to investigate the effect of ethanolic extract of Crescentia cujete leaves on MPP+ induced cell viability by MTT assay, SRB assay, Trypan blue exclusion assay, Lactate Dehydrogenase (LDH) and generation of reactive oxygen species (ROS) were measured in SH-SY5Y neuroblastoma cells. Results: The ethanolic extract of Crescentia cujete showed an IC50 of 159.29 μg/mL with R2 value of 0.947 in MTT assay, SRB assay showed an IC50 was 162.5μg/ml with R2 value of 0.884 and Trypan blue exclusion assay form a level of inhibition produced by different concentration increased with increasing concentration. Pre-treatment with the concentration of Crescentia cujete significantly diminished the release of LDH induced by MPP+ as LDH increases proportionally to the number of lysed cells and the ROS levels generated in response to ethanol extract of Crescentia cujete were significantly reduced in MPP+ treated SH-SY5Y cells than the standard Conclusions: The cell viability assays clearly show that the ethanol extract of Crescentia cujete leaves have the ability to protect SH-SY5Y cells against damage induced by MPTP and offer neuroprotection in vitro.

Keywords: Crescentia cujete, Parkinson's disease, SH-SY5Y Neuroblastoma cells, MPTP.