Abstract

HPTLC FINGERPRINTING AND PHARMACOLOGICAL EVALUATION OF EXTRACT OF PHYLLANTHUS FRATERNUS WEBSTER LEAVES

Santosh Vilas Gandhi*, Purnima Vishwanath Shinde, Aakanksha Avinash Dube

Abstract

The objective of present investigation was to develop HPTLC fingerprinting and evaluate analgesic activity of pet ether and methanolic extract of Phyllanthus fraternus webster leaves in rats. Extraction method used was maceration. Three solvents were used for extraction of phytoconstituents (pet ether, methanol and hydromethanolic). Various mobile phase were tried and the optimized mobile phase was n-Hexane : ethylacetate (6:4) v/v. used for fingerprinting. Analgesic activity of both extract of Phyllanthus fraternus Webster leaves was tested at a dose of 500 mg/kg & 1000 mg/kg. Activity was evaluated by Digital randall selitto and tail immersion method using Wistar rats of either sex (n = 6 in each group). The results indicated that the methanolic extract exhibited significant analgesic activity. It may be due to presence of saponin, steroids & alkaloids in it.

Keywords: Phyllanthus fraternus webster, HPTLC fingerprinting, Digital randall selitto, tail immersion method, analgesic activity.