Abstract

A NOVEL UPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF ABAMECTIN AND PRAZIQUANTEL IN BULK AND PHARMACEUTICAL DOSAGE FORM

Syeda Sana Saima* and Iffath Rizwana

Abstract

A new simple, accurate, precise, and robust Ultra performance liquid chromatography (UPLC) was developed and validated for simultaneous estimation of Abamectin and Praziquantel. The separation was achieved by using the combination of Acetonitrile: water: methanol in ratio (40:30:30) in zodiac C18 columnC18 (250×4.6 ID× 5μm) with a Flow rate of 1.0 ml/min at a wavelength of 244nm. Abamectin and Praziquantel were eluted at the retention time of 2.3 and 4 mins respectively. System suitability parameters are found to be within limits. Method produces linear responses within the concentration range 1535μg/ml for Abamectin and 40-120μg/ml for Praziquantel. The method was found to be accurate as the percentage recovery was 99.6 % and 98 % for ABM and PRQ and was within the limits. LOD was found to be 0.21μg/ml for abamectin and 2.09μg/ml for praziquantel LOQ was found to be 0.65μg/ml for abamectin and 6.35μg/ml for praziquantel. Proposed method was found to be simple, accurate, precise, and quick could be used for regular analysis.

Keywords: UPLC, Abamectin, Praziquantel.