Abstract

PROTEIN MARKER IDENTIFICATION OF VERATOXIN FROM CLINICALLY ISOLATED ESCHERICHIA COLI

Aseer Shakir Ajel, Ganesh Dama, Prasad M.P, Balasubramanian Sathyamurthy*

Abstract

This investigation was carried out to identify the protein marker for veratoxin from clinically isolated E.coli samples. The E.coli samples were isolated from clinical samples like blood, urine and stool of infected patients. The E.coli samples were conformed by the growth of the bacteria on EMB agar medium where they showed metallic sheath. The E.coli samples were grown on nutrient broth for the isolation of the protein. The proteins were precipitated using ammonium sulphate. The proteins got precipitated at 50% saturation of ammonium sulphate. The eluted proteins were then identified using SDS-PAGE with standard protein marker. Three E.coli samples isolated showed the band with 32,000KDa determing the presence of veratoxin protein in the E. coli samples.

Keywords: E.coli, Clinical samples, Veratoxin protein, SDS-PAGE.