Abstract

PRELIMINARY PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT OF ASTERCANTHA LONGIFOLIA (HYGROPHYLLA SCHULLI) ROOTS ALONG WITH VARIOUS ACTIVITIES

Shraddha R. Samrit*, Nidhita S. Kamble, Akanksha Dambhare, Atul T. Hemke, Milind J. Umekar

Abstract

Hygrophila spinosa T Ander is a promising medicinal plant with great economic potential. Its medical values are also appreciated in the ancient medical literature. The present work was carried out to investigate the Antioxidant, Antibacterial, Cytotoxic and Antiinflammatory activities of Astercantha Longifolia (Hygrophylla Schulli). Disc diffusion assay, DPPH assay, brine shrimp assay and rat paw edema test were performed to check the activities respectively. The TLC Fingerprinting of ethanolic root extract shows presence of compound whose structure was established as Stigmast-5-en-3 β-0l which is responsible for anti-inflammatory activity and its activity was checked using carraageenan induced rat paw edema model. The Extract produced significant antibacterial activity against Bacillus subtillus, Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli with zone of inhibition of 20, 16, 18 and 18 mm respectively. The extract showed a significant free radical scavenging activity with an IC50 of 88.06μg/mL. The extract showed significant lethality to brine shrimp lethality using Vincristine sulfate as standard with LC50 value of 56.37 μg/mL.

Keywords: Astercantha Longifolia, Hygrophylla Schulli, Antioxidant, Antibacterial, Cytotoxicity and Anti-inflammatory activity.