Abstract

DEVELOPMENT AND VALIDATION OF UVSPECTROPHOTOMETRIC METHOD FOR ESTIMATION OF TANGERETIN IN ORANGE PEEL POWDER

Sachin Bhusari*, Amit Rindhe and Pravin Wakte

Abstract

Aim: To develop and validate a simple, precise and cost effective UVvisible spectrophotometric method for the estimation of tangeretin in Orange Peel Powder extracts. All the parameters of the analysis were chosen according to ICH Q2 (R1) guideline. Methods: Tangeretin solution was scanned over UV-visible range for its wavelength of maximum absorbance. Various calibration standards of tangeretin were prepared. Calibration curve of concentration vs. absorbance was plotted. Various analytical method validation parameters were calculated. Results: The maximum wavelength of tangeretin was found to be 327 nm. The correlation coefficient at concentration range of 0.5-10 μg/ml was found to be 0.9999. The validation study of the developed UV method was carried out by conducting linearity, accuracy, precision, robustness, ruggedness, limit of detection and limit of quantitation studies. Developed UV method was found to be precise for the intra and inter day studies and showed standard deviation in the range of 0.12 to 1.56 & 0.35 to 1.71 respectively. The total percent recovery of tangeretin was found to be 98.29 to 100.02%. Conclusion: A simple, precise and cost-effective UV- visible spectrometry method was developed for the estimation of tangeretin in standardized extract of Powder of Orange Peel. The said method was developed using solvent containing economical percentage of organic phase in aqueous media. Said validated UV- visible method can be efficiently used for the estimation of tangeretin in extracts of Orange Peel Powder.

Keywords: UV- Visible Spectrometric Method, Tangeretin, Orange Peel, Validation.