Abstract

PHYTOCHEMICAL EVALUATION AND HPTLC FINGER PRINTING PROFILE OF LEAVES OF FILICIUM DECIPIENS

Kavitha S.* and Parvathavardhini D.

Abstract

A plant derivative plays a vital role in the modern pharmaceuticals for various ailments. High performance thin layer chromatography (HPTLC) is a valuable technique for the investigation of plant products with respect to different aspects of their quality. The present study was carried out to investigate the secondary metabolites and to develop HPTLC. To study the HPTLC fingerprinting of leaf extract of Filicium decipiens, A CAMAG HPTLC system equipped with LINOMAT 5 applicator and WIN CATS-1.3.4 software were used. The Phytochemical tests was done on methanol, ethanol and chloroform extracts. Preliminary Phytochemical studies shows the presence of various phytoconstituents like alkaloids, saponins, flavonoids, phenols and tannins and the three solvent extract were used for the further analysis. HPTLC study revealed the presence of more bands in chloroform than methanol and ethanol extract of Filicium decipiens. The HPTLC fingerprinting profile developed for chloroform extract will help in proper identification and quantification of marker compounds. By isolating and identifying marker compounds, it can be used for the formulation of new drugs to treat various diseases.

Keywords: Filicium decipiens, HPTLC, fingerprinting, phytochemical screening.