Abstract

EFFECTS OF SOME ANTIOXIDANT VITAMINS AND CHELATING AGENT ON BIOCHEMICAL ALTERATIONS IN HYPERCHOLESTEROLEMIC RATS.

Arafa, M.M, Aly S. A. and Shaheen, N.E*

Abstract

A total number of sixty adult male Wistar rats were used in this studyto investigate the protective effects of vitamin C (vit C),vit E andEDTA alone and their combinations on hypercholesterolemia throughmarkers of lipid profile, thyroid function, some liver andoxidativeenzymes. Rats were randomly divided into six groups(n = 10). Group I: normal control, Group II: hypercholesterolemic rats,Groups III: received ethylenediaminetetraacetic acid (EDTA)(1gEDTA/kg 1g/Kg), group IV: received 1gml/Kg body weight of vitC, group V: received vit E1g/Kg feed and Group IV received EDTA,vit C and vit E. The results of this study showed that rationsupplemented with EDTA, VC and vit E either individual or in aCombination group induced significant decrease (P<0.05) in total cholesterol (TC),triglyceride (TG), low-density lipoprotein (LDL) and very low-density lipoprotein (VLDL)levels in hypercholesterolemic rats. Also, atherogenic index (AI), lipid peroxidation(MDA),thyroid stimulating hormone (TSH), the activities of serum aspartate and alanineaminotransferases (AST, ALT), alkaline phosphatase (ALP) gamma-glutamyltransferase(GGT) levels recorded significant decrease, but high-density lipoprotein (HDL), superoxidedismutase (SOD), reduced glutathione(GSH), serum triiodothyronine (T3) and thyroxine (T4)exhibited significant increase. Moreover, the administration of EDTA tohypercholesterolemic rats (group III) causes significant decrease in serum calcium (Ca),phosphorus (P), magnesium (Mg) and iron with significant increase in total iron bindingcapacity (TIBC) in comparison with other groups. The overall result, the improvement beingmore in rats treated with mixture of EDTA, vit C and vit E The present results suggested that supplementation with EDTA, vit C and vit E attenuated most of the changes induced in hypercholesterolemic rats by feeding rats with cholesterol-rich diet owing to their observed anti-hyperlipidemic and antioxidant properties. In conclusion, the present study provides obvious evidence that both vit C, vit E and EDTA hold the promise of being useful hypocholesterolemic, and peroxidative agent and should be administered to the body continuously, targeted to the biological site susceptible to oxidative damage.

Keywords: Hyperlipidemia oxidative stress, EDTA and antioxidant Vitamins.