Abstract

DETECTION OF DENGUE VIRUS FROM F1 GENERATION OF FIELD COLLECTED LARVAE FROM DWARKA AND ROHINI, DELHI” AT NATIONAL INSTITUTE OF MALARIA RESEARCH (ICMR).

*Nilu Kumari, Dr. Himmat Singh, Dr. Amit Kumar Pandey, Dr. Sneha Pandey

Abstract

Dengue is one of the most dangerous infectious disease and itemerging as the major public health concern in various parts of India.The aim of our study was to detect dengue virus in F1 generation offield collected larvae from Dwarka and Rohini, New Delhi. Wecollected larvae from different inside and outside placed containers onthe basis of their characteristics and selection of habitat from differentlocalities of Dwarka and Rohini. 10 or less than 10 mosquitoes wereused as a pool. Then, these mosquitoes undergo the RNA-Extractionmethod by using TRIZOL. The collected RNA samples were thentested for DENV presence using RT-PCR Techniques. RT-PCR is themodification of PCR techniques and is used to amplify a singlestranded RNA template to yield abundant double stranded complimentary DNA products.After RT-PCR, the the PCR product is separated through AGAROSE GELELECTROPHORESIS and the sample runs on the solidified surface of agarose and theyvisualize under the GEL DOCUMENTATION on the basis of their band formation at BasePAIR ABOVE 500, detected as the positive sample, confirms the presence of dengue virus inthe mosquito of BAGHDOLA, Dwarka, Delhi represented positive result of our study.

Keywords: Dengue, AGAROSE GEL ELECTROPHORESIS, Aedes aegypti, Aedes albopictus.