Abstract

EVALUATION OF ANTIOXIDANT AND ANTICANCER ACTIVITY OF AYAPANA TRIPLINERVIS

Asha Mary John*, Sunitha Thomas, Emilia Anil, Musallama K. Jabbar, Thejes Antony and Remya Gayathri S.

Abstract

Cancer is a major health problem worldwide. Antioxidants are free radical scavengers and plays a significant role in treatment of cancer. Ayapana triplinervis is a tropical American shrub in the family Asteraceae. Various plant derived products like terpinods, flavonoids, steroids, essential oils etc. have been noted in Ayapana triplinervis and it have wide variety of pharmacological actions. This thesis attempts to evaluate the invitro antioxidant and anticancer activity of leaves of Ayapana triplinervis. Invitro antioxidant activity was screened by standard ABTS method using ascorbic acid as standard and anticancer activity was investigated by standard MTT assay method using HeLa (cervical cancer) cells. Preliminary phytochemical evaluation of ethanolic and aqueous leaf extracts of Ayapana triplinervis showed the presence of alkaloids, aminoacids, carbohydrate, flavonoids, saponin glycosides and steroids. Both the extract exhibits a dose dependent activity. In ABTS method maximum percentage of inhibition was shown at concentration 2000mcg/ml. The viability of the cells was evaluated by MTT assay method and alcoholic extract at concentration 100mcg/ml showed maximum percentage of inhibition. From the study it was concluded that the leaves of Ayapana triplinervis may be considered as a good remedy for oxidative stress and cancer.

Keywords: Ayapana triplinervis, anticancer, antioxidant, MTT assay, ABTS method.