Abstract

HYALURONIDASE (HAASE) INHIBITERS IN HUMAN SERUM

Sawsan Arfan Maaroof*

Abstract

The main target of this study was to investigate the natural compounds effecting on a Hyaluronidase enzyme (HAase) behaviour on human serum for creating new inhibitors to be used then as medications. In this work the activities of HAase enzyme were measured using Alfred Linker methods after mixed it separately with (i) 3,4-Diyhdroxy-3 chloro flavylium chloride, (ii) 3,4-diyhdroxy-3-acetoxy flavylium chloride) and (iii) heparin compounds at different concentrations: (1x10-1,1x10-2,1x10-3,1x10-4) μM and incubation period times (0-25) minutes. The experimental works exhibited that, these compounds affecting on HAase enzyme activity in human serums from 60 to 90 % for 10 minutes’ incubation period time and the competitive inhibition rates were (III>II>I)

Keywords: Hyaluronidase, enzyme, and incubation.