Abstract

PHARMACOLOGICAL EVALUATION OF ANXIOLYTIC ACTIVITY OF CANNABIS SATIVA EXTRACT IN ALBINO MICE

Dheeraj Dubey*, Avadh Biharee, Arpita Yadav, Mohit Shrivastava and Dr. Kamal Kishore Maheshwari

Abstract

The present work was performed to evaluate the anxiolytic activity in mice of ethanolic extract of cannabis sativa. The anxiolytic activity was evaluated by marble burying apparatus. Anxiolytic activity of cannabis sativa extract was compared with diazepam. Physically induced depression parameters showed various behavioural abnormalities in experimental animals. Mice were divided into 5 groups of 6 animals in each group. In marble burying apparatus Group I- Normal saline (as per body weight), Group II- Diazepam (2mg/kg), Group III-Cannabis sativa (10 mg/kg), Group IV-Cannabis sativa (20 mg/kg), Group V-Cannabis sativa (40 mg/kg). Analyzed the animals for anxiolytic effect of Cannabis sativa extracy by using marble burying apparatus. The result showed that Cannabis sativa extract at gradually increasing concentration 10 mg/kg, 20 mg/kg and 40 mg/kg significantly decreases marble burried in marble burying apparatus. Thus, Cannabis sativa extract may be included in effective treatment strategy of anxiolytic disorder.

Keywords: Anxiolytic activity, Cannabis sativa, Marble burying apparatus, Diazepam.