Abstract

HPTLC METHOD DEVELOMENT FOR THE ESTIMATION OF EUPALITIN-3-O-B-D-GALACTOPYRANOSIDE FROM TABLET AND HERB

Ashvini Maval* and Dr. A. V. Baviskar

Abstract

A simple, rapid, selective and quantitative HPTLC method has been developed for determination of Eupalitin-3-O-B-D-galactopyranoside in Punarnava extract and Punarnava tablet formulation. The alcoholic extract of Boerhavia diffusa and its ayurvedic formulation‐Himalaya Punarnava tablet samples were applied on TLC Aluminium plate pre coated with Silica gel60 GF254 and developed using Ethyl acetate: Methanol:Formic acid (5:0.5:0.5) v/v as a mobile phase. Quantification was carried out densitometrically using an UV detector at wavelength of 366 nm. The results obtained complies the limit of assay as per I.P.

Keywords: Punarnava, Punarnava tablet, Eupalitin-3-O-B-Dgalactopyranoside, TLC, HPTLC.