Abstract

PHYTOCHEMICAL SCREENING AND IN-VITRO ANTIOXIDANT ACTIVITY OF ETHANOLIC LEAVES EXTRACT OF DETARIUM MICROCARPUM

Usman Garba Kurmi*, Babagana Modu, Parth Patel**, Fatima Abubakar, Ruqayya
Babagana Umar, Hadiza Abubakar Balkore, Ruqayya Ali Uthman, Prosper Obed
Chukwuemeka and Dattatreya Mukherjee

Abstract

Detarium microcarpum is generally utilized for the treatment of various diseases in Northern Nigeria. Using the spectrophotometer technique against 1,1-dipenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2), the phytochemical and antioxidant free radical rummaging behavior of the ethanolic leaves concentrate of this plant was examined in vitro. Free radical scavenging activity of the plant extract against DPPH and H2O2 was focus subordinate with IC50 estimation of 4.84 and 2.77 separately as contrasted and those of standard ascorbic acid with IC50 estimation of 2.93 and 1.72 individually. In this research, it was discovered that the ethanolic extract of D. microcarpum contains high phenolic content that may represent the powerful action against DPPH and H2O2 radicals. This scavenging action is presumably due to the presence of hydroxyl bunch joined to the aromatic ring structures and accordingly help to extinguish the radicals. The presence results show that leaves extract of D. microcarpum has strong in-vitro antioxidant activity because of the presence of phenolic mixtures. The findings suggest that ethanolic extract of D. microcarpum possess natural antioxidant property that could be used for treating several free-radical mediated diseases and also for herbal formulations.

Keywords: In vitro, antioxidant activity, phytochemicals screening, IC50, Detarium microcarpum, Ascorbic acid.