Abstract

MOLECULAR CHARACTERIZATION OF JASMINE SPECIES BY RAPD MOLECULAR MARKERS

Sushant Shekhar and Prasad M.P*

Abstract

In the following study genomic DNA was extracted from eightJasmine species. DNA fingerprinting was performed by the randomamplified polymorphic DNA (RAPD)-PCR. RAPD has been one of themost commonly used molecular techniques to develop DNA markerssince it does not require prior knowledge of a DNA sequence. RAPDPCRproduced a spectrum of amplification products which arecharacteristics of the selected Jasmine DNA. From the DNAfingerprinting, Dendrogram was constructed and genetic similaritymatrix were estimated which revealed variations between selectedspecies of Jasmine. A total of 15 random primers were used for conducting the RAPDanalysis. The primers were OPZ5, OPZ6, OPZ7, OPZ8, OPZ9, OPZ10, OPZ11, OPZ12,OPZ13, OPZ14, OPZ15, OPZ18, OPZ19, OPZ20, OPZ21 and OPZ22. Of these selectedrandom primers OPZ8, OPZ9, OPZ10 produced clear banding patterns which were used forconstructing Dendrogram. OPZ8 produced a total 58 bands ranging from 7-11, OPZ9produced a total 91 bands ranging from 10-13 and OPZ 10 produced a total 49 bands rangingfrom 5-8 for eight jasmine samples. Most of the bands were monomorphic with somepolymorphic bands which can be used for marker development for these jasmine species. Thedescribed approach holds great promise for genetic diversity polymorphism, cultivarcharacterization and genetic population conservation of Jasmine species.

Keywords: Jasmine, DNA fingerprinting, RAPD, Genetic Diversity.