WJPR Citation New

  All Since 2011
 Citation  2903  2393
 h-index  27  24
 i10-index  68  60

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

ACETONE, THE THIRD KETONE BODY. II. ACTIONS ON ERYTHROCYTE MEMBRANE AND HEMOGLOBIN IN TOTAL HUMAN BLOOD

José D. Méndez* and Edith García-Pérez

Abstract

Previously, was informed by our group that glycated hemoglobin was produced in hemoglobin samples incubated with acetone and β- hydroxybutyrate alone, suggesting that glycated hemoglobin can be non-enzymatically formed in vitro from ketone bodies in the absence of carbohydrates, providing evidence that ketone bodies contribute to the development of complications of un-controlled type 2 diabetes and mainly of type 1 diabetes. In this study, the effect of acetoacetate and acetone at concentrations similar to those found in ketoacidosis was studied in total human blood. Blood samples (3 mL) of 48 volunteers were grouped and treated as follow: 1. D-glucose (Control), 2. Acetoacete, 3. D- glucose plus acetoacetate, 4. Acetone. In order to inhibit glycation, glycine, glycilgycine, urea, aminoguanidine and Larginine were added. Samples were made in duplicate with their respective controls and incubated in the dark for 90 days at 37º C. Concentrations of hemoglobin and glycated hemoglobin were measured at the start and at the end of the study. Glycated hemoglobin was determined by turbidimetric immunoassay using specific antibodies and by fluorescence testing using two pairs of filters: Excitation 320/emission 460 nm and excitation 355 / emission 460 nm. High concentrations of glucose produced glycation of hemoglobin in intact erythrocytes. Acetoacete did not show relevant glycation of hemoglobin in the conditions of this study. When acetone was added to the blood samples, an inmediate denaturing effect on plasma proteins was observed and erythrocyte membrane disaggregation occurred. Glycation by glucose was inhibited by L-arginine while no inhibitory effect on hemoglobin glycation by glycine, glycylglycine, aminoguanidine and urea was observed. In conclusion, as expected major glycation of hemoglobin was observed when total blood was exposed to high concentration of glucose. No relevant glycation was observed with acetoacetate. Disaggregation of the membrane erythrocyte and denaturing effect on proteins was caused by acetone. Additional studies are necessary to evaluate the effect of different concentrations of acetone on the integrity of the erythrocyte membrane, as well as other blood cells and subcellular organelles, and on plasma proteins in short periods of time.

Keywords: Human Erythrocyte, Diabetes Mellitus, Ketone Bodies, Acetoacetate, Acetone, Advanced Glycation end Products, L-arginine, Aminoguanidine, L-glycine, Glycilglycine, Urea.


[Full Text Article]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More