STRUCTURAL ELUCIDATION OF BIOACTIVE COMPONENTS IN AQUEOUS EXTRACT OF Eugenia uniflora LEAVES USING FTIR ANALYSIS
*Dr. Geedhu Daniel
Abstract
The isolation of active plants compounds lead to discover a several
drugs derived directly from plants. The present study aims at structural
elucidation of bioactive compounds in aqueous hot extract of Eugenia
uniflora leaves using FTIR analysis. 10 mg of the dried extract powder
was encapsulated in 100 mg of KBr pellet inorder to prepare
translucent sample discs. The powdered sample of plant specimen was
loaded in FTIR spectroscope (Shimazdu, IR affinity 1, Japan) with a
scan range from 400 to 4000 cm-1 with a resolution of 4cm-1. FTIR
analysis of the extract revealed the presence of different functional
groups at different peaks. Screening of functional groups represents the
presence of alkanes, aliphatic amines, alcohols, carboxylic acids,
amines. The presence of these functional groups is indicating various
medicinal properties of Eugenia uniflora. From the result it can be
concluded that the medicinal properties of the extract may be due to the presence of these
functional groups. However, advanced spectroscopic studies are required for structural
elucidation and identification of the active principles in the extract.
Keywords: FTIR, Eugenia uniflora, aqueous hot extract.
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