Abstract

ISOLATION, SCREENING AND CHARACTERIZATION OF CELLULOLYTIC BACTERIA FROM COTTON GINNING INDUSTRY EFFLUENT POLLUTED SOIL SAMPLES

Kondakindi Venkateswar Reddy*, Pillalamarri Ramu, Afreen Fathima and Pabbati Ranjit

Abstract

Release of industrial liquid waste on to the cultivated lands causes a significant change in biogeochemical cycling and primal matter processing. The present study, cotton ginning industry liquid waste discharged soil (Test) and unexploded soil (Control) were taken from the neighbouring area of the cotton ginning mill, the test and control soil samples were used for the analysis of physico-chemical and biological properties of the soil sample. Further, the test soil samples were used as a source of inoculum for cellulolytic bacteria by using serial dilution and agar plating method. For the isolation and screening of cellulolytic bacteria, selective media that is CMC agar was used. All the isolates were analyzed morphological, physiological, and biochemical characterization, based on analysis, the isolates resembled to Bacillus subtilis and Bacillus cereus.

Keywords: Polluted soil samples, physico-chemical and biological properties, CMC agar, Cellulolytic Bacteria.