Abstract

PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF LEAF EXTRACT OF SPHAGNETICOLA TRILOBATA

Sulekha Afzal and Rajesh B. R.*

Abstract

Plant derived medicine are widely used in traditional cultures all over the world their use ranges the production of mainstream pharmaceutical products to herbal medicine preparations. The result of the study revealed that the secondary metabolites such as alkaloids, saponins, flavanoids, phytosterol, phenol and tannins, terpenoids, coumarins, cardiac glycoside and carbohydrates were present in the leaf extracts of Sphagneticola trilobata. The maximum phenolic content was found to in the aqueous extract (57.93mg/g) of Sphagneticola trilobata. The maximum flavanoid content was found in the ethyl acetate extract of Sphagneticola trilobata. In DPPH free radical scavenging assay, the mean IC50 values of methanolic extracts and ethyl acetate extract of Sphagneticola trilobata was lower which showed that the radical scavenging activity was showed to be effective in these solvent extracts (53.41 and 70 μg/ ml respectively). In in-vitro antidiabetic assay, it was found that the methanolic extract of Sphagneticola trilobata showed significant activity.

Keywords: Sphagneticola trilobata, Antidiabetic activity, DPPH assay, Phytochemicals.