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Abstract

ANALYTICAL METHOD DEVEOPMENT AND VALIDATION USING RP-HPLC METHOD FOR SOME ANTICANCER DRUG

P. S. Kalantri* and S. G. Jawarkar

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Abstract

A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validation of Plerixafor. Chromatography was carried out on aXterra RP 18 (4.6 x 250mm, 5μm) column using a mixture of Methanol: Water (50:50% v/v) as the mobile phase at a flow rate of 0.8ml/min, the detection was carried out at 215nm. The retention time of the Plerixafor was 5.481 min respectively. The method produce linear responses in the concentration range of 10-50mg/ml of Plerixafor. The method precision for the determination of assay was below 2.0%RSD. The method is useful in the quality control of bulk and pharmaceutical formulations.

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