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Abstract

INGREDIENTS IDENTIFICATION, PHYSICO-CHEMICAL AND HPTLC EVALUATION OF PRAJASTHAPAN CHOORNA

Grishma Ninama* and Shilpa B. Donga

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Abstract

Background: Prajasthapana Choorna is a combination of herbal drugs having a numerous number of references in Ayurveda classics. But its qualitative data hasn’t been explored thoroughly yet. It is mainly useful to treat gynaecological diseases, infertility, repeated abortion and in diseases where according to Ayurveda Vata and Pitta Doshas are vitiated. Aim: To develop pharmaceutical profile of Prajasthapana Choorna. Material and Method: Study included preparation of Prajasthapana Choorna following all SOPs using raw drugs, which were previously authenticated. Later, Prajasthapana Choorna was subjected to physicochemical and high performance thin-layer chromatography (HPTLC) analysis as per standard protocols. Result and Discussion: Pharmaceutical analysis showed that the loss on drying value was 0.40%, ash value was 9.56%w/w, water soluble extract was 7.24%, methanol soluble was 10.22%, acid insolubleash 0.76%, and pH is 4.5. Methanolic extract of Prajasthapana Choorna shows presence of alkaloids, flavonoids, phenols, tannin, sugar, steroids, saponin, cardiac glycosides and carbohydrates which can be hypothesized for its estrogenic activity. HPTLC fingerprinting profile of Prajasthapana Choorna revealed 5 spots at 254 nm and 5 spots at 366nm. Conclusion: The present investigation will be helpful in assessing the pharmacognostical, phytochemical analysis and laying down pharmacopoeial standards for Prajasthapana Choorna.

Keywords: HPTLC, Prajasthapana Choorna, Phytochemical, Prajasthapana Gana.


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