Abstract

A REVIEW ON LC- MS TECHNIQUE AND IT'S APPLICATION

Zeel Shah*, Hardi Patel, Janvi More, Mitali Dalwadi, Chainesh Shah and Umesh Upadhayay

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Abstract

Chromatography is a separation technique used to separate the respective compound from a mixture using a stationary Phase and mobile phase. Introduction of chromatography is a millstone event in biomedical research. Chromatographic separation is based on the basis of adsorption, partition, ion exchange, molecular exclusion, affinity and Chirality. Liquid Chromatography/Mass spectroscopic analysis (LC/MS) is quick developing and it's the well-liked appliance of liquid chromatographers. Liquid chromatography-mass spectrometry (LCMS/ MS) is a method that uses liquid chromatography (or HPLC) with the mass spectrometry. The Liquid Chromatography-Mass Spectrometry (LC-MS) is an effective analytical method with very high sensitivity and specific. Mixture of chromatography with spectrometry is initial announced in 1967 and initial LC-MS system was introduced in 1980s. It has been continuously used in development of drug at many different stages including Metabolic assurance Screening, Metabolite Identification as well as In Vivo Drug Screening, Impurity Identification, Peptide tracing, Glycoprotein Mapping, Natural Products Dereplication, Bioaffinity Screening. LC-MS is now applied successfully to daily analysis in many fields, consisting therapeutic drug monitoring (TDM), clinical and forensic toxicology as well as adultrantrate control. This advancement in LCMS was initially and still is sustain by the demand for extra powerful analytical and bio-analytical methods that can accurately and precisely differentiate target analytes from huge complexity mixtures in a conscious and selective way. With recent advances in instrumentation, the use of liquid chromatography (LC) and mass spectrometry (MS) has become an effective two-dimensional (2D) hyphenated technology.

Keywords: Chromatography, Liquid Chromatography/Mass Spectroscopy (LC/MS), Liquid Chromatography (HPLC), Mass Spectroscopy (MS).