Abstract

ASSESSMENT OF ANTIBACTERIAL, ANTI-INFLAMMATORY AND ANTIOXIDANT EFFECTS OF CARICA PAPAYA - AN IN-VITRO STUDY

Dr. Salman Khawar*

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Abstract

Aim: To assess the antibacterial, anti-inflammatory and antioxidant activity of Carica papaya pulp extract under laboratory condition. Method: In the present study, the methanolic extract of Carica papaya pulp was assessed for its antibacterial effects against S.mutans, S.sanguis and S.aureus using agar disc diffusion method and minimum inhibition concentration was determined by micro broth dilution technique. The anti-inflammatory effect was assesses by lipoxygenase inhibition assay and hyaluronidase inhibition assay. The antioxidant effects were assessed by ABTS 2, 2’-azino-bis (ethylbenzothiazoline-6-sulphonic acid) radical scavenging assay and 2, 2 diphenyl–1 picrylhydrazyl (DPPH) antioxidant assay. This was done in triplicates for each concentration of the extracts. The statistical analysis was done by Student unpaired t-test. Results: The methanolic extract of Carica papaya pulp extract showed no inhibitory effects on S.mutans, S.sanguis and S.aureus. The test results of DPPH assay revealed that the quercetin group had higher radical scavenging property as compared to Carica papaya group and the difference was statistically significant. The results obtained from the ABTS assay revealed that the quercetin group had higher radical scavenging property with as compared to Carica papaya. The difference in results obtained were statistically significant.(p

Keywords: Periodontitis, Carica papaya, lipoxygenase, hyaluronidase, antioxidant, anti-inflammatory, DPPH, ABTS.